About

A Molecular Plant Pathologist with more than ten years of experience in plant virology.

Skills

  • bioinformatics
    5
  • Molecular biology
    9
  • Lab management
    7
  • virology
    9
  • English
    10
  • Kiswahili
    10
  • Latin
    1

Experience

Kinoga Michael

Work experience
  • Marketing events to potential exhibitors and participants.
  • Following up on the invited exhibitors and participants to ensure they register via the provided registration links.
  • Compile the lists of confirmed attendees.
  • Maintaining and ensuring proper recording of contact details of all institutions engaged for exhibitions and the individual participants.

Kinoga Michael

Work experience
  • December, 2018 - September, 2023
  • Fulltime
  • Managed a whole project on identifying tree tomato viruses, as well as the molecular aspect of a project on Identification of fungal pathogens associated with bulb onion post- harvest rots.
  • Development of virus diagnostics assays for papaya and tree tomato viruses, identification of Fusarium sp. using sequence analysis.
  • Testing for seed transmission of viruses in papaya and tree tomato.
  • Sequencing via the Ion Torrent NGS platform.
  • Development of a working DNA extraction protocol for the fall armyworm.
  • Trained bioinformatician in NGS data analysis and Bash scripting.
  • Trained in conventional and quantitative LAMP assays in sweet potato, vaccine development, ELISA kit development.

Kinoga Michael

Work experience
  • January, 2018 - December, 2018
  • Fulltime
  • Training of biotechnology diploma students on molecular plant work including, plant tissue culture, PCR, ELISA, operation of different lab equipment etc.

Kinoga Michael

Work experience
  • September, 2016 - January, 2017
  • Fulltime
  • Identification of pathogens affecting banana plants using symptoms and molecular methods.
  • Establishing a working nucleic acid extraction protocol from banana.
  • Keeping good records, inventories and making requisitions for depleted chemical stocks and reagents.
  • Virus indexing using conventional and RT- PCR.
  • Developing and testing SOP’s for use and maintenance of equipment.
  • Ensure that there is flow of information from the molecular lab to the tissue culture section.

Kinoga Michael

Education
  • January, 2016 - September, 2023
  • Fulltime
  • Thesis: Occurrence and distribution of viruses infecting tree tomato
  • (Solanum betaceum Cavs) in Kenya
  • Kinoga, M. et al., 2022. Genome characterisation of two complete coding sequences of tomato mild mottle virus from tree tomato and their distribution in Kenya. Journal of Plant Pathology.
  • Kinoga, M., Kuria, P., Miano, D. N. R. & Wasilwa, L., 2021. First report of Ethiopian tobacco bushy top virus and its associated satellite RNA in mixed infection with Potato virus Y infecting Solanum betacea in Kenya. New Disease Reports, 44 (e12015).
  • Kinoga, M., Kuria, P., Miano, D. & Wasilwa, L., 2021. First report of Potato spindle tuber viroid infecting tree tomato in Kenya in mixed infection with Potato virus Y. New Disease Reports, 44(e12029).

Kinoga Michael

Education
  • Certificate
  • Second Class Honours (Upper division)

Kinoga Michael

Work experience
  • DNA and RNA extraction from cassava leaves followed by PCR detection of CMV and identification of markers for resistance to CMV and CBSV.
  • Seed viability testing.
  • Trouble shooting contamination in citrus tissue culture.
  • Teaching undergraduate and masters students molecular techniques.

Kinoga Michael

Work experience
  • DNA and RNA extraction, PCR, and RCA technology in screening for Banana streak viruses (BSV).
  • Marker assisted breeding in the characterisation and selection of Jojoba (Simmondsia chinensis) using SSRs and Banana.
  • Scoring and data collection for stem rust of wheat.
  • Provide technical assistance and training to students and visiting scientists at the centre.

Kinoga Michael

Work experience
  • DAS ELISA for the detection of viruses causing maize lethal necrosis disease (MCMV SCMV) in Kenya.
  • qPCR for the detection of Rift valley fever virus (RVFV).
  • DNA extraction from cassava leaves and RT-PCR in a project that established the expression of β-carotene in the leaves of genetically modified cassava.

Kinoga Michael

Work experience
  • Clinical Chemistry- operations of Cobas Integra and AC5 Diff.
  • PCR and DAS ELISA for the detection of HIV from serum.
  • Specimen reception, aliquoting, PBMC separation and haematology.
  • Laboratory database and documentation.

Kinoga Michael

Work experience
  • Use of PCR based techniques in detection of point mutations in P. falciparum that are associated with resistance; in Diagnosis of Wuchereria bancrofti using urine, sputum, serum/blood and mosquitoes.
  • Flow cytometry using 4 colour FACS Calibur and FACS Count, preparation of culture media, lymphocytes proliferation assays/cells culture, haemotological differentiation of cells by staining/cell counting techniques.
  • Parasitology techniques: Kato-Kaz techniques for diagnosis of S. mansoni; perfusions; DNA extraction methods from worms followed by PCR; culturing of leishmania and malaria parasites, counting, staining, and measuring infection of sand flies and mosquitoes with parasites; microscopy techniques.
  • Rearing of phlilebotomine sandflies, identification of sandflies using sandfly keys, preparation of larval food and throughout the life cycle of a sandfly.
  • Animal care and handling.
  • Training in Good Laboratory Practice (GLP).

Kinoga Michael

Work experience
  • The following are the duties that were assigned to me during the attachment
  • Field visits, sampling, and data collection.
  • Detection sweet potato viruses from leaf samples using Nitrocellulose membrane ELISA.
  • Detection of Ralstonia solanacearum from potato tubers through Double antibody sandwich ELISA.
  • Tissue culture techniques in virus cleaning and micro-propagation.
  • Capture, identification, and preservation of insects and mites.

Kinoga Michael

Education
  • Bachelor of Science
  • Second class honours (Upper division)