About

I am seeking a postdoctoral position in structural biology, with a particular focus on protein-DNA interactions and drug discovery-related research.

Skills

  • Communication
    8
  • Teamwork
    9
  • Problem-Solving
    9
  • Creativity
    9
  • Leadership
    9
  • protein biochemistry
    9
  • structure biology
    9
  • structural bioinformatics
    9
  • English
    8

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Experience

Praveen Rai

Work experience
  • Focus: Quantification and expression of anti-apoptotic proteins of Leishmania parasite for developing novel therapeutics.
  • Research Responsibilities
  • Leishmania culture, animal cell culture, etc.
  • Molecular cloning of anti-apoptotic genes, expression and purification of anti-apoptotic protein using different chromatographic techniques, and knockout generation.
  • Structural and Biochemical characterization of anti-apoptotic protein.

Praveen Rai

Work experience
  • Focus: Structural and Biochemical characterization of plant-specific structure selective DNA resolvases.
  • Research Responsibilities
  • Proficiently achieved molecular cloning, active site mutant generation, protein truncations, and expression and purification of various truncated WT and active site mutant and full-length recombinant DNA resolvases in E. coli, involving large-scale culture preparation, affinity, charge, and size-based column chromatography, as well as crystallization plate setup.
  • Conducted crystal fishing and crystal diffraction data collection and diffraction data analysis to further understand the DNA resolvases' characteristics.
  • Performed comprehensive biochemical characterization of DNA resolvases to gain insights into their enzymatic binding property with branched DNA substrate.
  • Contributed to the preparation of research manuscripts intended for publication in esteemed scientific journals.

Praveen Rai

Education
  • August, 2015 - February, 2022
  • Fulltime
  • Molecular modeling of primase protein, virtual screening of lead compound against primase, MD simulation of selected lead compound through Gromacs MD simulation software followed analysis, etc.
  • Leishmania culture, animal cell culture, etc.
  • Molecular cloning primase gene, expression and purification of primase protein using different chromatographic techniques.
  • High throughput assays and inhibition assays of primase protein.
  • Parasite inhibition assay.

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