DERRICK MULOMA

at***ck@gmail.com

8/2/2022

"A biochemist experienced in Molecular biology and phylogenetic "

Work experience

▪ Molecular identification of novel coronavirus, COVID-19: RNA extraction, real-time RT-PCR, and Rapid calorimetric LAMP detection for identification of coronavirus and specifically COVID-19.
▪ Preventive maintenance of equipment
▪ General sample handling: reception and preparation of saliva, nasopharyngeal, and/or oropharyngeal swabs for molecular analysis
▪ Results analysis, reporting, and recording for COVID-19

Work experience

▪ Molecular identification of novel coronavirus, COVID-19: RNA extraction and real-time RT-PCR for identification of coronavirus and specifically COVID-19
▪ Preventive maintenance of equipment
▪ General sample handling: reception and preparation of nasopharyngeal and/or oropharyngeal swabs for molecular analysis.
▪ Results analysis, reporting, and recording for COVID-19 samples

Work experience

 -General sample handling: sample reception and storage of nasopharyngeal swab specimen
 - Molecular identification and characterization of respiratory viruses: nucleic acid extraction, real time RT- PCR for identification of influenza A and B among other respiratory viruses
 -Nucleic acid quantification using Nanodrop spectrophotometer and cubit fluorometer
 -Agarose gel electrophoresis
 -Sanger’s chain termination sequencing technique
 -culture of respiratory viruses using mammalian continuous cell lines
 -Isolation of respiratory viruses from specific cell lines
 -Preparation of media reagents for cell culture and virus transportation
 -clean cell culture techniques; cryopreservation, revival and cell passaging of mammalian cells.
 -Serological characterization of influenza virus using Hemagglutination, Hemagglutination inhibition and Immunofluorescence assays
 -Recording and reporting all results to data manager
 -Training and orientation of new attaches and interns in the laboratory
 -preventive maintenance of equipment